On 20 February, the second round of the Europa League knockout play-offs was completed and the 16 finalists for this season's Europa League were announced. The list of qualified teams is as follows:

2024/25 Europa League top 16

Teams that directly qualified in the league stage: Lazio, Athletic Bilbao, Manchester United, Tottenham Hotspur, Frankfurt, Lyon, Olympiakos, Glasgow Rangers

Teams that qualified in the knockout play-offs: Bodø/Glimt, FCSB, Ajax, Real Sociedad, Roma, Viktoria Plzen, AZ Alkmaar, Fenerbahçe

Potential Europa League quarter-final matchups

1st Lazio, 2nd Athletic Bilbao will face 15th Roma, 16th Viktoria Plzen

3rd Manchester United, 4th Tottenham Hotspur will face 13th Real Sociedad, 19th AZ Alkmaar

5th Frankfurt, 6th Lyon will face 11th FCSB, 12th Ajax

7th Olympiakos, 8th Glasgow Rangers will face 9th Bodø Glimt, 24th Fenerbahçe